Bridge expansion joints play a crucial role in bridges. In order to solve problems such as the relatively high maintenance costs of traditional expansion joints, an assembled and quickly installed multi-directional displacement bridge expansion joint (hereinafter referred to as the ZPF assembled expansion joint) was now proposed. According to the “General Technical Requirements for Highway Bridge Expansion Joints” (JT/T 327 - 2016), on-site fatigue tests were designed, and finite element analysis was also carried out at the same time. The test results show that the fatigue life of the ZPF assembled expansion joint decreases as the fatigue load increases. The fatigue life of the ZPF assembled expansion joint depends largely on the concrete under the embedded steel plate. During the loading of the fatigue performance test for 2 million cycles, the overall structure is safe and stable without any abnormal phenomena. The research results can provide a basis for the application of the ZPF assembled expansion joint.