At the present stage in China, the situation of rural domestic sewage collection and treatment is grim, and management is difficult. Rural domestic wastewater treatment has become one of the important tasks in the implementation of rural revitalization strategy. Focusing on rural MBR membrane treatment stations, the influence of water quality on their operating conditions was comprehensively analyzed. Key water quality parameters including chemical oxygen demand (COD), ammonia nitrogen (NH? - N), total phosphorus (TP), and suspended solids (SS) were analyzed in detail to clarify the characteristics of wastewater quality. In terms of sludge characteristics, the capillary suction time (CST) was used to evaluate the sludge dewatering performance, and it is found that the change of water quality could change the difficulty of sludge dewatering. Using bioinformatic analysis, it is revealed that the structure of microbial community was intrinsically linked to water quality parameters, and the relative abundance of species under different water quality conditions showed obvious differences and was closely related to the treatment efficiency. With the help of membrane fouling monitoring methods, such as transmembrane differential pressure (TMP) measurement, the membrane fouling status can be grasped in real time. Through correlation analysis, it is found that there are complex associations between water quality parameters, sludge characteristics and biological information, which affect the operation of rural MBR membrane treatment stations, providing a comprehensive theoretical basis and data support for the precise control of the operation of the stations, which can help to improve the efficiency and quality of rural wastewater treatment and reduce the operating costs.