Based on the quasi-natural experimental conditions provided by three batches of smart city pilot policies, and on the basis of comprehensively measuring the resilience level of urban pharmaceutical manufacturing industry, the panel data of 54 prefecture-level cities from 2009 to 2019 was used to explore the impact of smart city pilot construction on the resilience of municipal pharmaceutical manufacturing industry by using multi-period DID (difference in differences) model. The results show that the pilot construction of smart city can significantly promote the resilience of urban pharmaceutical manufacturing industry. Mechanism analysis shows that the pilot construction of smart city promotes the resilience of pharmaceutical manufacturing industry through technological innovation, while the level of information infrastructure construction has a positive moderating effect.