In order to realize the construction of China's "major and regional communication are fully connected" air transport service network, promote the coordinated development of China's major and regional airlines, and create convenient conditions for people's travel, the evolutionary game model between major airlines and regional airlines is constructed based on the evolutionary game theory. The stability conditions and stability of major airlines, regional airlines and major and branch intermodal transport systems are discussed, and the strategic choices of both sides of the game in the evolution process are studied. The results show that the choice of the cooperative strategy of the system is related to the position of the saddle point, the closer the position of the saddle point is, the greater the probability that both parties choose not to cooperate. When the closer the position of the saddle point is to the non-cooperative strategy, the greater the probability that both parties choose to cooperate.