Through a questionnaire survey of 320 employees from different industries, the effect of information overload on employee burnout, the mediating role of job anxiety, and the moderating mechanism of leader-member exchange was explored. The findings show that information overload has a significant positive relationship with the three dimensions of burnout (emotional exhaustion, depersonalization of dehumanization, and personal achievement),job anxiety plays a mediating role in the effects of information overload on the three dimensions of burnout,and the leader-member exchange not only negatively moderates the indirect effects of information overload on burnout through job anxiety, but also negatively moderates the direct effects of information overload on the depersonalization of dehumanization.