In order to explore the current situation and problems of agricultural development in Lishu County and analyze its total agricultural output value, this paper adopts the empirical research method of multiple linear regression and uses the software SPSS 22.0 to select the data of total agricultural output value of Lishu County and related influencing factors from the databases of Jilin Provincial Bureau of Statistics and National Bureau of Statistics of China during 2003-2020. Taking total agricultural output value as dependent variable and rural population, total power of agricultural machinery, effective irrigation area, fertilizer application amount (material amount) and total sown area of cities and counties as independent variables, the above influencing factors were analyzed quantitatively. The results showed that when the total power of agricultural machinery increased by 10,000 kW, the total agricultural output value decreased by 19,779.5 million yuan on average. Every increase of 1000 hectares of effective irrigated area will increase the total agricultural output value by 74.54066 million yuan on average. Every increase of 1 hectare in the total sown area of cities and counties will increase the total agricultural output value by 53,311 yuan on average.