With the Incites and ESI analysis platform as the data source, combined with the bibliometric analysis method and EXCEL software, the multi-dimensional benchmarking analysis on the international competitiveness of ESI potential disciplines and superior disciplines of 27 agricultural universities in China was conducted, providing data reference for promoting and improving the discipline development and construction of agricultural universities and the international competitiveness of disciplines. The research shows that China Agricultural University, Nanjing Agricultural University, Huazhong Agricultural University and South China Agricultural University have the strongest comprehensive discipline strength. Agricultural universities with relatively weak comprehensive discipline strength mainly come from non 211 and non 985 universities in Yunnan, Inner Mongolia, Xinjiang and other provinces; Botany and zoology, agricultural science, environment and ecology, biology and biochemistry, chemistry and engineering are the main superior disciplines in agricultural universities; A single discipline indicator is not enough to comprehensively evaluate the international competitiveness of the discipline. The key to improving the global average level of cited performance of scientific research institutions is to combine multiple indicators such as the number of papers, cited frequency, CNCI value, high cited papers and their proportion, the number of papers cited to the top 1%, and the number of papers cited to the top 10%; It is still a powerful measure to enhance the international competitiveness of agricultural colleges and universities by promoting the development of weak disciplines and potential disciplines with superior disciplines, tapping outstanding researchers in superior disciplines, and increasing cooperation with high-level research institutions at home and abroad.