According to the characteristics of the single base linear route structure in the production and operation of domestic small airlines, It is the first time to combine pilot fatigue with crew scheduling,based on the weekly flight plan, the traditional crew scheduling assignment model is further improved and innovated, and the flight crew scheduling assignment model based on fatigue equilibrium is studied. First, quantify the flight fatigue of the crew and determine the calculation model of pilot fatigue.The second is to build a crew scheduling and assignment model based on fatigue equilibrium.Thirdly, genetic algorithm is adopted to solve the problem.Fourth, take the flight plan of existing airlines as an example, and compare and analyze the fatigue degree of pilots on that day with the original scheduling. The results show that the fatigue degree of pilots has been greatly improved, and the difference between the fatigue degree of each pilot and the average fatigue degree of the day is small, and the fatigue degree of pilots is relatively stable, so the study of crew assignment model based on fatigue equilibrium is true and effective.