As an important part of provincial science and technology innovation base, provincial key laboratory is an important carrier of implementing innovation driven development strategy, and provides strong scientific and technological support for economic and social development. Gansu Province has made remarkable achievements in laboratory classification, layout, management and operation by optimizing and integrating provincial innovation base platform in recent years. It is a complex system composed of government, scientific research institutes, universities, enterprises and researchers. The provincial key laboratory performance evaluation research is conducted, the key laboratory performance evaluation indicators are established, analytic hierarchy process is used to determine the weight of indicators, fuzzy comprehensive evaluation is conducted to build a performance evaluation model, the analysis of the results of the model is conducted..Reasonable suggestions are put forward to provide important for the benign development of the laboratory in the new period In order to standardize the laboratory operation and improve the quality of laboratory construction.