In order to study the anisotropy characteristics of the mechanical strength of layered rock masses, seven kinds of limestone samples with different bedding dip angles were drilled indoors, and uniaxial compression tests were carried out, supplemented by acoustic emission monitoring. The test results show that: As the bedding dip angle increases, the uniaxial compressive strength of the limestone presents a "V"-shaped characteristic that first decreases and then increases, and reaches the minimum value when the bedding dip angle is 45°.Taking 45° as the boundary, before 45°, the acoustic emission energy accumulation curve has two obvious steps. There is a sharp increase at the two points of 0.3σp (σp is the peak intensity) and 0.7σp. The acoustic emission energy accumulation curve is at 45° and beyond. It increases in a zigzag shape, without obvious steps. The b value of acoustic emission is bounded by 45°. Before 45°, the overall trend is downward, and there is a step in the middle that rises and maintains a step. After 45°, it is in a "V" shape, The lowest point appears at about 0.6σp degrees.