Aiming at the transfer of tacit knowledge in the process of technology transfer, combined with the related evolutionary game theory, the method of copying dynamic equations is used to analyze its influencing factors, the equilibrium situation and the strategy choices of both parties in the process of tacit knowledge conversion. The results of the analysis show that the greater the amount of tacit knowledge possessed, the ability to impart knowledge, the ability to absorb knowledge, the willingness to transfer knowledge, the willingness to accept knowledge, and the degree of knowledge transferability, the greater the possibility of both parties in technology transfer choosing tacit knowledge transfer; The higher the absorption cost, risk cost, and speculative return, the greater the possibility that both parties of technology transfer will choose not to transfer tacit knowledge. Taking into account the above influencing factors,countermeasures and suggestions are put forward to promote the completion of the transfer of tacit knowledge by both parties of technology transfer.