In this paper, 21 prefecture-level cities in Guangdong Province are studied, and the spatial and temporal characteristics of regional innovation output in Guangdong Province from 2007 to 2018 are analyzed by means of variation coefficient, standard deviation and exploratory spatial analysis. The results showed that:①Between 2007 and 2018, the overall level of regional innovation output in Guangdong Province increased, and the absolute difference of innovation output between regions increased, and the relative difference decreased.②During the study period, there was a clear polarization in the distribution of spatial density in guangdong province, and there was a gradual trend of increasing.③In the spatial distribution, the regional innovation output in Guangdong Province shows a concentrated trend, mainly in the Pearl River Delta region of high-value aggregation, Guangdong West, Guangdong North, Guangdong East low-value dispersion, as low-value areas surrounded by high-value areas of the distribution trend, knowledge spillover effect is obvious.