Based on the statistical analysis of the data of the fourth national economic census of Yangzhou, this paper explores the current situation of R & D input-output of industrial enterprises in Yangzhou, summarizes the advantages and existing problems of R & D of industrial enterprises in Yangzhou, and puts forward suggestions to enhance the R & D Input-output Performance Effect of industrial enterprises：effectively increase the R & D investment intensity, increase the input intensity of basic research, focus on attracting and retaining talents, and build a scientific and technological level Taiwan integrates innovative resources, thus helping Yangzhou to build a new scientific and technological innovation city.