As the center of airline operation and management, dispatch release work plays a decisive role in aviation safety. This paper used DEMATEL-ISM method to model and analyze the influence of human factors in dispatch release. Firstly, based on the existing research and discussion with experts, the human factors system in dispatch release was constructed. Then the relationship between the factors was found out and the hierarchical structure of the factor system was divided. Finally, put forward suggestions for improvement. The results show that rules and regulations and education and training are essential factors, and the key factors are the same as enterprise philosophy. In the factor system, they are closely related to other factors and affect other factors, and have a higher degree of importance.