Analyzed the interest conflict of hotel management in the process of college-enterprise cooperation and the influence mechanism of stakeholders through questionnaires, in-depth interviews and text analysis on the basis of stakeholder theory. It was found that the interests of stakeholders was an important factor affecting the college-enterprise cooperation; wide gap from the expectations before practice and satisfaction after practice was the mainly conflict of interests for students; the conflicts for college and hotel were mainly included that students hoped to learn knowledge, skills and accepted training but hotel wanted to through at low cost to obtain human labor and performance, students need respected and recognized the value identity but imperfect management from hotel; college’ public nature but hotel performance and pursuit profit, college focus on improvement of students training and comprehensive quality but hotel was apt obtain cheap labor not much training were the mainly conflicts for college and hotel. Finally put forward to constructed effective communication and coordination mechanism, to meet the interests of the three aspects of the reasonable demands, and improve the college-enterprise cooperational security system.