Promoting the work of enterprise-university-scientific research cooperation, meets the urgent need for universities to serve local economic and social development, and also meets the inherent requirement to promote the development of universities itself. In this paper, take the scientific and technological service activities for practice, the modes to carry out the work of enterprise-university-scientific research cooperation were introduced, the main problems of enterprise-university-scientific research cooperation existed in agricultural universities were analyzed, and effective measures and advices to strengthen this work were proposed.