Based on the relevant indicators’ statistical data of sci-tech output from 2000 to 2011 of Wuhan and national central cities, which consist of Beijing, Chongqing, Tianjin, Shanghai and Guangzhou, we made a comparative analysis of the output of sci-tech work in this paper. All these data demonstrated the current situation and significant differences in their output of sci-tech work between Wuhan and national central cities. In the end, through analyzing the significant reasons to the differences, the reasonable suggestions were put forward to improve the sci-tech output capability of Wuhan, which could be a reference for the future development, communication and cooperation.