Quantitative Evaluation of regional integration process and the intensity of Industry linkage was basis and precondition for improving regional competitiveness, optimizing the allocation of resources. Based on spatial shift-share analysis model, quantitative analysis was used to evaluate the industrial structure evolution and competitive trend in the 6 cities along the Shanghai-Nanjing high-speed rail that was located in the Yangtze River Delta core area. The results shows that: Strengthening the economic links of the Nanjing, Shanghai and other 4cities, become the core of both ends along the Shanghai - Nanjing high speed rail; Most of the cities in the region's industrial sector growth momentum is large, having strong spatial industry competitiveness; Urban industrial structure continues to be optimized, but there are also regional industry competitiveness issues of decline, the industrial division of labor is not clear.