To realize the production-education integration, these two problems, whom to cooperate with and where to start from, should be firstly solved. Therefore, the method of selection of cooperation partners and starting points based on optimal single index principle was proposed in this paper. The global selection models of cooperation partners and starting points were built. The evaluation index systems for enterprises and universities were established respectively. The Analytic Hierarchy Process was applied to determine the weight of each index, the Questionnaire Research was used to score the qualitative index, the Standard Score theory was applied to standardize the original datas of quantitative index. The implementation procedure to select the cooperation partners and starting points based on optimal single index principle was carried out finally by an example. The research results can open a new way and provide a wide research space for selection of cooperation partners and starting points in the production-education integration.