Using the method of vertical load increment, combined with statistical analysis of aircraft turbulence data and reanalysis data, the spatiotemporal distribution characteristics of aircraft turbulence on four high-altitude routes in 2014 was investigated. The results indicate that the turbulence frequency on the high-altitude routes exceeded 55%, reaching as high as 78.8% on the Lhasa route. Turbulence encounters are more frequent during the departure and arrival phases compared to the cruising phase. The occurrence of aircraft turbulence shows a seasonal variation, with lower frequencies in the summer and fall seasons and higher frequencies in the winter and spring seasons. During the cruising phase, aircraft turbulence primarily occurs in the altitude range of 6 900~8 700 meters. In contrast, during the departure and arrival phases, the frequency of turbulence encounters increased as the altitude decreased. The spatial distribution of aircraft turbulence exhibits an elongated shape, with high-frequency turbulence areas observes in the 91~93 °E and 97~99 °E regions of the ＺＵＬＳ, as well as the 31~33°N region of ＺＵＪＺ.